FIGURE 3 from Inhibition of Microsomal Prostaglandin E2 Synthase Reduces Collagen Deposition in Melanoma Tumors and May Improve Immunotherapy Efficacy by Reducing T-cell Exhaustion

Collagen deposition and distribution of tumor-infiltrating immune cells in tumors derived from ptgs2-KO and ptges-KO murine BrafV600E melanoma cells. A, Left, Representative images of Masson's trichrome staining of tumors derived from scramble, ptgs2-KO, and ptges-KO cells. Scale bar = 100 μm. A, right, Collagen positivity (%) was automatically calculated using Visiopharm software. B–G, Distribution of tumor-infiltrating immune cells was compared between tumors from scramble, ptgs2-KO, and ptges-KO cells. The number of tumor-infiltrating immune cells was automatically calculated using Visiopharm software. Shown are the numbers of tumor-infiltrating CD8a+ cytotoxic T cells (B), tumor-infiltrating GrB+ effector CD8a+ T cells (C), tumor-infiltrating Tim3+ exhausted CD8a+ T cells (D), tumor-infiltrating classic CD8a+ DCs (E), tumor-infiltrating NK1.1+ NK cells (F), and tumor-infiltrating CD19+ B cells (G). Two to five ROIs, depending on tumor size, were randomly selected from each tumor and used for analyses. Graph values represent mean ± SD. Significance in difference between two groups was determined by Student t test. **, P P < 0.05.