Protein precursor features and amino acid conservation of novel linear helminth AMPs.

Signal peptide region indicated in red, predicted AMP region indicated in green and other peptides shown in blue. Scissors icon denotes region of putative cleavage sites. Note that boxes are not drawn to scale. Predicted AMP peptide conservation graphic shown below was generated using WebLogo3 (www.weblogo.threeplusone.com; [62, 63]) using a multiple sequence alignment generated using Clustal Omega [29] of A) 21, B) 6, C) 5, D) 3, E) 11 peptides. WebLogo is coloured by amino acid chemistry [Green = Polar, Neutral = Purple, Basic = Blue, Acidic = Red, Hydrophobic = Black]. The height of amino acid letters indicates probability of an amino acid at a specific location. The consensus peptide sequence with gaps removed was used to obtain peptide charge and hydrophobic % using the APD3 Peptide Calculator and Predictor (https://aps.unmc.edu/prediction/predict; [23]).