S1 Fig -

Construction strategy for P. oxalicum mutants involving introduction of truncated cxrA genes into ΔcxrA;G418R+ (A) and confirmation by PCR analysis (B-E). (B) PCR production of POX_d05452 with primers POX_d05452-F/POX_d05452-R. (C) DNA fragment with primers POX_d05452-LF/Ble-VR. (D) DNA fragment with primers Ble-VF/POX_d05452-LR. M: 1 kb DNA marker; 1: ddH2O; 2: Δku70;hphR+, 3: CcxrA;bleR+, 4: 1–60; 5: 1–206; 6:1–591; 7: 17–733; 8: 61–733; 9: 61–206. (E) PCR verification of mutant R94A;bleR+. M: 1 kb DNA marker; 1: ddH2O; 2: Δku70;hphR+, 3: R94A;bleR+. Left panel indicates amplification of DNA fragment with primers POX_d05452-F/POX_d05452-R; Middle panel shows PCR products with primers POX_d05452-LF/Ble-VR; Right panel shows PCR amplification of DNA fragment with primers Ble-VF/POX_d05452-LR. The bottom panel shows verification of DNA sequence. M: 1 kb DNA marker; 1–12: Transformants; +: Δku70;hphR+;–: ddH2O. (TIF)