We develop magnetic cytoskeleton affinity (MiCA) purification, which allows for rapid isolation of molecular motors conjugated to large multivalent quantum dots, in miniscule quantities, which is especially useful for single-molecule applications. When purifying labeled molecular motors, an excess of fluorophores or labels is usually used. However, large labels tend to sediment during the centrifugation step of microtubule affinity purification, a traditionally powerful technique for motor purification. This is solved with MiCA, and purification time is cut from 2 h to 20 min, a significant time-savings when it needs to be done daily. For kinesin, MiCA works with as little as 0.6 μg protein, with yield of ∼27%, compared to 41% with traditio...
The biomolecular motor protein kinesin and its associated filamentous protein microtubule have been ...
A cargo encounters many obstacles during its transport by molecular motors as it moves throughout th...
With their longest dimension typically being less than 100 nm, molecular motors are significantly be...
We develop magnetic cytoskeleton affinity (MiCA) purification, which allows for rapid isolation of m...
The field of microfluidics has drastically contributed to downscale the size of benchtop experiments...
Recent developments in optical microscopy and nanometer tracking have facilitated our understanding ...
Recent developments in optical microscopy and nanometer tracking have facilitated our understanding ...
Rigid attachment of microtubules (MTs) to glass cover slip surfaces is a prerequisite for a variety ...
Motor proteins, like kinesin, transport cargo within biological cells by transforming chemical energ...
We have developed a novel, microfabricated, stand-alone microfluidic device that can efficiently sor...
Numerous microtubule-associated molecular motors, including several kinesins and cytoplasmic dynein,...
Cytoskeletal motors transform chemical energy into mechanical work to drive essential cellular funct...
Cytoskeletal motors transform chemical energy into mechanical work to drive essential cellular funct...
iii The separation of duplicated chromosomes during mitosis is achieved by the mitotic spindle which...
Molecular motors, essential to force-generation and cargo transport within cells, are invaluable too...
The biomolecular motor protein kinesin and its associated filamentous protein microtubule have been ...
A cargo encounters many obstacles during its transport by molecular motors as it moves throughout th...
With their longest dimension typically being less than 100 nm, molecular motors are significantly be...
We develop magnetic cytoskeleton affinity (MiCA) purification, which allows for rapid isolation of m...
The field of microfluidics has drastically contributed to downscale the size of benchtop experiments...
Recent developments in optical microscopy and nanometer tracking have facilitated our understanding ...
Recent developments in optical microscopy and nanometer tracking have facilitated our understanding ...
Rigid attachment of microtubules (MTs) to glass cover slip surfaces is a prerequisite for a variety ...
Motor proteins, like kinesin, transport cargo within biological cells by transforming chemical energ...
We have developed a novel, microfabricated, stand-alone microfluidic device that can efficiently sor...
Numerous microtubule-associated molecular motors, including several kinesins and cytoplasmic dynein,...
Cytoskeletal motors transform chemical energy into mechanical work to drive essential cellular funct...
Cytoskeletal motors transform chemical energy into mechanical work to drive essential cellular funct...
iii The separation of duplicated chromosomes during mitosis is achieved by the mitotic spindle which...
Molecular motors, essential to force-generation and cargo transport within cells, are invaluable too...
The biomolecular motor protein kinesin and its associated filamentous protein microtubule have been ...
A cargo encounters many obstacles during its transport by molecular motors as it moves throughout th...
With their longest dimension typically being less than 100 nm, molecular motors are significantly be...