Add to ORKGDefining protein-protein interactions (PPIs) in their native environment is crucial to understanding protein structure and function. Cross-linking-mass spectrometry (XL-MS) has proven effective in capturing PPIs in living cells; however, the proteome coverage remains limited. Here, we have developed a robust in vivo XL-MS platform to facilitate in-depth PPI mapping by integrating a multifunctional MS-cleavable cross-linker with sample preparation strategies and high-resolution MS. The advancement of click chemistry-based enrichment significantly enhanced the detection of cross-linked peptides for proteome-wide analyses. This platform enabled the identification of 13,904 unique lysine-lysine linkages from in vivo cross-linked HEK 293 cells, ...
Chemical cross-linking coupled with mass spectrometry has emerged as a powerful strategy which enabl...
Chemical cross-linking coupled with mass spectrometry has emerged as a powerful strategy which enabl...
Chemical cross-linking coupled with mass spectrometry has emerged as a powerful strategy which enabl...
Protein-protein interactions (PPIs) are fundamental to the structure and function of protein complex...
International audienceChemical cross-linking (XL) coupled to mass spectrometry (MS) has become a pow...
: The majority of processes that occur in daily cell life are modulated by hundreds to thousands of ...
The function of a protein is mediated by its ability to form precise three-dimensional structures or...
Cross-linking mass spectrometry (XL-MS) has received considerable interest, owing to its potential t...
We describe an integrated workflow that robustly identifies cross-links from endogenous protein comp...
Recent development of mass spectrometer cleavable protein cross-linkers and algorithms for their spe...
Proteins are involved in almost all processes of the living cell. They are organized through extensi...
Chemical cross-linking coupled with mass spectrometry has emerged as a powerful strategy which enabl...
Chemical cross-linking coupled with mass spectrometry has emerged as a powerful strategy which enabl...
Chemical cross-linking coupled with mass spectrometry has emerged as a powerful strategy which enabl...
Chemical cross-linking coupled with mass spectrometry has emerged as a powerful strategy which enabl...
Chemical cross-linking coupled with mass spectrometry has emerged as a powerful strategy which enabl...
Chemical cross-linking coupled with mass spectrometry has emerged as a powerful strategy which enabl...
Chemical cross-linking coupled with mass spectrometry has emerged as a powerful strategy which enabl...
Protein-protein interactions (PPIs) are fundamental to the structure and function of protein complex...
International audienceChemical cross-linking (XL) coupled to mass spectrometry (MS) has become a pow...
: The majority of processes that occur in daily cell life are modulated by hundreds to thousands of ...
The function of a protein is mediated by its ability to form precise three-dimensional structures or...
Cross-linking mass spectrometry (XL-MS) has received considerable interest, owing to its potential t...
We describe an integrated workflow that robustly identifies cross-links from endogenous protein comp...
Recent development of mass spectrometer cleavable protein cross-linkers and algorithms for their spe...
Proteins are involved in almost all processes of the living cell. They are organized through extensi...
Chemical cross-linking coupled with mass spectrometry has emerged as a powerful strategy which enabl...
Chemical cross-linking coupled with mass spectrometry has emerged as a powerful strategy which enabl...
Chemical cross-linking coupled with mass spectrometry has emerged as a powerful strategy which enabl...
Chemical cross-linking coupled with mass spectrometry has emerged as a powerful strategy which enabl...
Chemical cross-linking coupled with mass spectrometry has emerged as a powerful strategy which enabl...
Chemical cross-linking coupled with mass spectrometry has emerged as a powerful strategy which enabl...
Chemical cross-linking coupled with mass spectrometry has emerged as a powerful strategy which enabl...