Principal component analysis (PCA) of observed concentrations of microbial targets excluding those classified in the third group (GI-FRNAPH-inf, GII-FRNAPH-inf, and PMMoV) in the samples (n = 94).

The analysis employs concentrations of microbial targets with 24% or higher positive rates excluding those classified in the third group by PCA shown on Fig 3 (i.e., HF183, Pig-2-Bac, GII-FRNAPH-gene, GIV-FRNAPH-gene, GIV-FRNAPH-inf, crAssphage, PMMoV, E. coli, FPH-plaque, FPH-MPN, and FDNAPH). Circles (○) and triangles (Δ) refer to the indicators quantified based on gene and viability, respectively. The vertical and horizontal axes indicate principal components (PC) 1 and 2, which explained 40.0% and 27.5% of the total information, respectively. (TIF)