Fig 1 -

Sensitivity of each species-specific TaqMan system: Mana-cdd (a) and Mana-ylxR (b) for M. anatis, Mans-dnaN for M. anseris (c) and Mclo-deoC for M. cloacale (d). Duplicate reactions were performed between 100 and 103 copy numbers. RFU: relative fluorescence units. The horizontal line at 100 RFU indicates the threshold for positivity. For results of further sensitivity tests repeated on different days see S5 Table.