Inhibiting lymphangiogenesis reduces T cell recruitment to the mLN.

(A) Experimental design. M.tb-infected mice were treated with MAZ51 i.p daily for 10 days, starting from day 11 post aerosol M.tb infection. TdTomato+ splenocytes were transferred 18 h before harvest. (B) Representative images of Lyve-1+ (green) in MAZ51- and vehicle-treated control mice (scale bar, 150 μm). (C) Proportion of Lyve-1+ region across entire area of MAZ51- and vehicle-treated control mice. Each symbol denotes an individual LN section (4–5 sections/mouse). (D) Total number of tdTomato+CD4+, CD8+ and B220+ lymphocytes in mLN of control and treated mice. (E) Total number of tdTomato+ CD69-CD44low naïve CD4+ and CD8+ T cells in mLN of control and MAZ51-treated mice p.i. (F) Number of total endogenous CD4+, CD8+ and B220+ lymphocytes in mLN of control and MAZ51-treated mice. (G) Number of endogenous ESAT64-17 specific (ESAT64-17:I-Ab) CD4+ T cells in mLN of control and MAZ51 treated mice. Each symbol represents an individual animal. Statistical differences between groups were determined using Student’s t test. *p p < 0.0001.