Claudin-1 localisation at the tight junctions is disrupted by PAF and restored by BML-111.

Composite X/Y confocal images of 16HBE14o- polarised monolayers untreated (control) or treated with P. aeruginosa filtrate (PAF) for 24h or PAF + 200 nmol/L BML-111 (BML) immunostained for (A) ZO-1 (red), (B) Claudin-1 (red), (C) E-cadherin (red). Nuclei were stained with Dapi (blue). All images were obtained at ×63 magnification. X/Z cross-sectional images are shown beneath with the apical surface oriented to the top. Scale bars (20 μm) are shown to the bottom left of the control image only but apply to all images. Graphical data of pixel intensities at the tight junctions for each protein are shown to the right of the images as means ± SD (bars) with individual data points. Non-parametric statistical analysis was carried out using nonparametric Kruskal‐Wallis multiple comparison test. Significant differences as shown, *P<0.05, all n = 3.