FIGURE 1 from Immuno-PET Imaging of CD69 Visualizes T-Cell Activation and Predicts Survival Following Immunotherapy in Murine Glioblastoma

ICIs influence T-cell distribution in tumor and spleen. Mice (n = 28) were inoculated with GL261 cells, treated with ICI or vehicle control, and analyzed at the designated time points relative to treatment. A, Schematic showing tumor inoculation day, treatment days, and time points relative to treatment in which mice were analyzed. B, Percentage of CD8+ TILs (red dots) and CD4+ TILs (blue dots) in each time point relative to treatment, for vehicle-control group (left panel) and ICI-treated group (right panel) C, Absolute number of TILs per mm3 of tumor. CD8+ cells × 102 (upper panels; red dots) and CD4+ cells × 102 (bottom panels; blue dots) in each time point relative to treatment, for ICI-treated group and vehicle-control group. D, Frequencies of CD8+ (red dots) and CD4+ T-cells (blue dots) in spleen in each time point relative to treatment, for vehicle-control group and ICI-treated group. B–D, Shown are means ± SEM. Scattered dots represent individual mice. Shown are representative data of two independent experiments. n = 3–4 per group. CD8+ and CD4+ were gated from CD3+CD45+ cells. One-way ANOVA with multiple comparison test (*, P P P <0.001).