KDM6A is required for highly pathogenic coronavirus infection in Huh7.5 cells.

(A) KDM6A expression in KDM6A polyclonal KO Huh7.5 cells. (B) KDM6A polyclonal KO Huh7.5 cells were infected with icSARS-CoV-2-mNeonGreen at an MOI of 1. Infected cells were imaged via fluorescence microscopy (left) and mNeonGreen expressing cell frequency was measured 2 dpi (right). Scale bar: 300 μm. (C) Huh7.5 cells were infected with SARS-CoV-2 at an MOI of 0.1 for 1 dpi. Virus titer was measured by plaque assays. (D) KDM6A polyclonal Huh7.5 E6 cells were infected with SARS-CoV-2 (left), HKU5-SARS-CoV-1-S (middle) and MERS-CoV (right) at an MOI of 0.2. Cell viability relative to a mock infected control was measured 3 dpi with CellTiter Glo. (E) KDM6A polyclonal Huh7.5 E6 cells were infected with VSV peudovirus (VSVpp): VSV-G, SARS-CoV-2-S (left), SARS-CoV-1-S (middle), and MERS-CoV-S (right). Luciferase relative to the VSVpp-VSV-G control was measured 1 dpi. Data were analyzed by one-way ANOVA with Tukey’s multiple comparison test. Shown are mean ± SEM. *p (TIF)