S12 Fig -

Comparison of Dscam1 phenotype–diversity correlations between our present study (A-C) and previous study [24] (D-F). These down panels (D-F) were drawn from data in previous study [24], which showed around 5,000 isoforms are sufficient to pattern all 3 neuron systems analyzed (panels D-F). However, our data indicated that up to 10,000 isoforms are required for normal axon patterning in MB and MS neurons (panels B, C), while 2,000 isoforms are sufficient for normal dendrite self/non-self discrimination (panel A). Our results demonstrate that previous reports [24] have overestimated the true number of isoforms required for normal dendrite self/non-self discrimination but have underestimated the true number of isoforms required for normal axon patterning in MB and MS neurons. Moreover, inconsistent with their notion that Dscam1 diversity function was independent of the identity of the isoforms [24], our present data indicated that the function of Dscam diversity was associated with specific exon clusters or isoforms. Data used to generate graphs can be found in S1 Data. Dscam1, Down syndrome cell adhesion molecule 1; MB, mushroom body; MS, mechanosensory; WT, wild type. (TIF)