MicroRNA-29 is an essential regulator of brain maturation through regulation of CH methylation

Acknowledgments We thank the members of the Deshmukh Lab for critical review of this manuscript. We also acknowledge Dr. Natallia Riddick, Viktoriya Nikolova, and Dr. Kara Agster at the UNC Mouse Behavioral Phenotyping Laboratory, for their technical assistance. We thank Mervi Eeva, Ying Li, and Bentley Midkiff at the UNC Translational Pathology Laboratory for expert technical assistance. We also appreciate the technical assistance provided by Janice Weaver and Carolyn Suitt at the UNC Animal Histopathology and the Center for Gastrointestinal Biology and Disease (CGIBD), respectively. The graphical abstract was created partly with BioRender.com. This work was supported by NIH, United States (GM118331 and AG055304 to M.D.). H.S. is a Howard Hughes Medical Institute Fellow of the Damon Runyon Cancer Research Foundation, United States (DRG-2194-14). J.M.S. and T.S.P. were supported by The Eunice Kennedy Shriver National Institute of Child Health and Human Development (U54HD079124) and NINDS (P30NS045892) of United States. The UNC Mouse Behavioral Phenotyping Laboratory is supported by a grant from the National Institute of Child Health and Human Development (NICHD), United States (U54-HD079124). The UNC Translational Pathology Laboratory is supported in part by grants from the NCI (5P30CA016086-42), NIH (U54-CA156733), NIEHS (5 P30 ES010126-17), UCRF, and NCBT (2015-IDG-1007) of United States. Author contributions V.S., A.N., and E.H. conducted most of the experiments with help as described here. H.S. and M.E.G. performed the whole-genome bisulfite sequencing and its analyses. J.M.S. and T.S.P. analyzed all RNA-seq data. C.L.K., M.K., P.S., and S.M.H. performed small RNA-seq and miRHub analysis. C.P., J.G., and E.A. helped with imaging and analysis of immunohistochemical stains. C.F. and M.B. helped manage the mouse colony. J.L. and Y.-W.H. generated the miR-29-floxed mice. S.M. conducted and analyzed many of the neurobehavioral assessments. V.S., M.E.G., and M.D. outlined the project. M.D. supervised the project. V.S., A.N., E.H., and M.D. produced the final version of the manuscript.Peer reviewedPublisher PD