Development of a competitive indirect enzyme-linked immunosorbent assay for screening phenylethanolamine A residues in pork samples

Phenylethanolamine A (PEA), a new alternative beta-agonist, has been illegally used in farming to promote the muscle growth in food-producing animals. In this study, a sensitive and convenient competitive indirect enzyme-linked immunosorbent assay (ciELISA) was developed for determination of PEA residues in pork samples. The produced antibody was highly specific to PEA and exhibited a negligible cross-reactivity toward some other beta-agonists. The developed technique was characterized by the limit of detection below 0.08 mu g kg(-1) and the IC50 value of 0.93 pmol mL(-1) (0.32 ng mL(-1)). Validation of the technique was done using artificially spiked and naturally contaminated pork samples. The recoveries ranged from 79.6 to 112.6 % for the samples spiked at levels of 0.1-5 mu g kg(-1) with the variation coefficients below 15 %. The analysis of naturally contaminated samples showed that the obtained data corresponded with the data obtained by the LC-MS/MS. The developed ciELISA was shown to be a feasible highly sensitive and specific screening tool for PEA residue analysis.Phenylethanolamine A (PEA), a new alternative beta-agonist, has been illegally used in farming to promote the muscle growth in food-producing animals. In this study, a sensitive and convenient competitive indirect enzyme-linked immunosorbent assay (ciELISA) was developed for determination of PEA residues in pork samples. The produced antibody was highly specific to PEA and exhibited a negligible cross-reactivity toward some other beta-agonists. The developed technique was characterized by the limit of detection below 0.08 mu g kg(-1) and the IC50 value of 0.93 pmol mL(-1) (0.32 ng mL(-1)). Validation of the technique was done using artificially spiked and naturally contaminated pork samples. The recoveries ranged from 79.6 to 112.6 % for the samples spiked at levels of 0.1-5 mu g kg(-1) with the variation coefficients below 15 %. The analysis of naturally contaminated samples showed that the obtained data corresponded with the data obtained by the LC-MS/MS. The developed ciELISA was shown to be a feasible highly sensitive and specific screening tool for PEA residue analysis.A