New cell line from adipopancreatic tissue of Atlantic herring Clupea harengus
- Langner, S.
- Rakers, S.
- Ciba, P.
- Petschnik, A.E.
- Rapoport, D.H.
- Kruse, C.
DOIAbstract
We report the isolation, cultivation, and cryopreservation of cells from adipopancreatic tissue of adult Atlantic herring Clupea harengus. The cell population was cultured for >1 yr and had a mean doubling time of 2 wk. Immunocytochemical analyses revealed that >50% of cells contained glial fibrillary acidic protein and pan-cytokeratins. Vigilin, a well-conserved protein related to mRNA transport, was found in only 11% of cells. Alpha smooth-muscle actin and stage-specific embryonic antigen 1 were even less abundant (<5%). Although little is known about tissue homeostasis and regeneration in adult C. harengus, the heterogeneity and long-term proliferation of the cell population described here suggest that it may contain stem cells or progen...
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