U937 JV9082

Legionella pneumophila JV9298 DotF-GFP<strong>Tilt Series Date:</strong> 2020-12-12</p> <strong>Data Taken By:</strong> Yuxi Liu</p> <strong>Species / Specimen:</strong> Legionella pneumophila</p> <strong>Strain:</strong> Philadelphia-1_Lp02</p> <strong>Tilt Series Settings:</strong> Single Axis, tilt range: (-60.0°, 60.0°), step: 2°, constant angular increment, dosage: 120.0 eV/Ų, defocus: -6.0 μm, magnification: 33000x. </p> <strong>Acquisition Software:</strong> Serial EM</p> <strong>Upload Method:</strong> rundir</p> <strong>Processing Software Used:</strong> imod, tomo3d</p> <strong>Collaborators and Roles:</strong> Sample preparation, data collection & processing: Yuxi Liu</p> <strong>Purification / Growth Conditions / Treatment:</strong> 1h infection, grids coated with 25ug/mL vitronectin</p> <strong>Sample Preparation:</strong> Quantifoil R2/2 gold finder grid, 100% humidity, 22deg C, manual blotting</p>Files available via S3 at https://renc.osn.xsede.org/ini210004tommorrell/tomography_archive/ylx2020-12-12-5</p>U937_9082_tomo_5_bin2_SIRT_1k.rec, Reconstruction (Pixel Size 1.06 nm), 4.1 GB <a role="button" class="ui compact mini button" href="https://renc.osn.xsede.org/ini210004tommorrell/tomography_archive/ylx2020-12-12-5/3dimage_123619/U937_9082_tomo_5_bin2_SIRT_1k.rec" > <i class="download icon"></i> Download </a></p> U937_9082_tomo_5_bin2.st, Tilt Series (Pixel Size 0.53 nm), 695.3 MB <a role="button" class="ui compact mini button" href="https://renc.osn.xsede.org/ini210004tommorrell/tomography_archive/ylx2020-12-12-5/rawdata/U937_9082_tomo_5_bin2.st" > <i class="download icon"></i> Download </a></p&gt