JV9082 U937 infection

Lp DotF-GFP JV9082 infecting U937<strong>Tilt Series Date:</strong> 2020-10-29</p> <strong>Data Taken By:</strong> Yuxi Liu</p> <strong>Species / Specimen:</strong> Legionella pneumophila</p> <strong>Strain:</strong> Philadelphia-1_Lp02</p> <strong>Tilt Series Settings:</strong> Single Axis, tilt range: (-54.0°, 54.0°), step: 2°, constant angular increment, dosage: 100.0 eV/Ų, defocus: -5.0 μm, magnification: 33000x. </p> <strong>Microscope:</strong> Caltech Titan Krios</p> <strong>Acquisition Software:</strong> Serial EM</p> <strong>Upload Method:</strong> pipeline</p> <strong>Processing Software Used:</strong> Raptor</p> <strong>Purification / Growth Conditions / Treatment:</strong> JV9082 O.D.=2.5, U937 72h post TPA treatment.</p> <strong>Sample Preparation:</strong> Quantifoil R2/2 gold finder grid, 10nm gold, 95% humidity, 37 deg C, manual double blotting</p>Files available via S3 at https://renc.osn.xsede.org/ini210004tommorrell/tomography_archive/ylx2020-10-29-3</p>Lp_1_sec2.mrc, Tilt Series (Pixel Size 0.265 nm), 2.6 GB <a role="button" class="ui compact mini button" href="https://renc.osn.xsede.org/ini210004tommorrell/tomography_archive/ylx2020-10-29-3/rawdata/Lp_1_sec2.mrc" > <i class="download icon"></i> Download </a></p> Lp_1_sec2_part55_8.rec, Reconstruction (Pixel Size 1.06 nm), 1.0 GB <a role="button" class="ui compact mini button" href="https://renc.osn.xsede.org/ini210004tommorrell/tomography_archive/ylx2020-10-29-3/3dimage_114948/Lp_1_sec2_part55_8.rec" > <i class="download icon"></i> Download </a></p&gt