PW 1305

Pseudomonas aeruginosa strain PW 1305. Acquired from the Transposon mutant collection. (F7 CheW PW1305 phoAwp03q4D05).<strong>Tilt Series Date:</strong> 2015-02-23</p> <strong>Data Taken By:</strong> Ariane Briegel</p> <strong>Species / Specimen:</strong> Pseudomonas aeruginosa</p> <strong>Strain:</strong> </p> <strong>Tilt Series Settings:</strong> Single Axis, tilt range: (-60.0°, 60.0°), step: 1°, constant angular increment, dosage: 160.0 eV/Ų, defocus: 10.0 μm, magnification: 34000x. </p> <strong>Acquisition Software:</strong> UCSFTomo</p> <strong>Upload Method:</strong> pipeline</p> <strong>Processing Software Used:</strong> Raptor</p> <strong>Purification / Growth Conditions / Treatment:</strong> Streak on LB plates and incubate overnight at 37°C- Inoculate 5 mL MOPS Minimal Media Limited Nitrogen from plate and grow at 30°C for ~24 hours</p>Files available via S3 at https://renc.osn.xsede.org/ini210004tommorrell/tomography_archive/aba2015-02-23-5</p>PseudomonasdeltaF7CheWTM0005.mrc, Tilt Series (Pixel Size 0.32 nm), 3.4 GB <a role="button" class="ui compact mini button" href="https://renc.osn.xsede.org/ini210004tommorrell/tomography_archive/aba2015-02-23-5/rawdata/PseudomonasdeltaF7CheWTM0005.mrc" > <i class="download icon"></i> Download </a></p> PseudomonasdeltaF7CheWTM0005_full.rec, Reconstruction (Pixel Size 1.28 nm), 712.7 MB <a role="button" class="ui compact mini button" href="https://renc.osn.xsede.org/ini210004tommorrell/tomography_archive/aba2015-02-23-5/3dimage_52192/PseudomonasdeltaF7CheWTM0005_full.rec" > <i class="download icon"></i> Download </a></p&gt