PW3654

Pseudomonas aeruginosa, strain PW3654.Acquired from the Transposon mutant collection. PW3654- lacZwp06q3 (CheW of F6 system)<strong>Tilt Series Date:</strong> 2015-02-23</p> <strong>Data Taken By:</strong> Ariane Briegel</p> <strong>Species / Specimen:</strong> Pseudomonas aeruginosa</p> <strong>Strain:</strong> </p> <strong>Tilt Series Settings:</strong> Single Axis, tilt range: (-60.0°, 60.0°), step: 1°, constant angular increment, dosage: 160.0 eV/Ų, defocus: 10.0 μm, magnification: 34000x. </p> <strong>Microscope:</strong> Caltech Polara</p> <strong>Acquisition Software:</strong> UCSFTomo</p> <strong>Upload Method:</strong> pipeline</p> <strong>Processing Software Used:</strong> Raptor</p> <strong>Purification / Growth Conditions / Treatment:</strong> Streak on LB plates and incubate overnight at 37°C- Inoculate 5 mL MOPS Minimal Media Limited Nitrogen from plate and grow at 30°C for ~24 hours</p>Files available via S3 at https://renc.osn.xsede.org/ini210004tommorrell/tomography_archive/aba2015-02-23-45</p>PseudomonasdeltaFF6CheWNEWFEB0002.mrc, Tilt Series (Pixel Size 0.32 nm), 3.4 GB <a role="button" class="ui compact mini button" href="https://renc.osn.xsede.org/ini210004tommorrell/tomography_archive/aba2015-02-23-45/rawdata/PseudomonasdeltaFF6CheWNEWFEB0002.mrc" > <i class="download icon"></i> Download </a></p> PseudomonasdeltaFF6CheWNEWFEB0002_full.rec, Reconstruction (Pixel Size 1.28 nm), 712.7 MB <a role="button" class="ui compact mini button" href="https://renc.osn.xsede.org/ini210004tommorrell/tomography_archive/aba2015-02-23-45/3dimage_52271/PseudomonasdeltaFF6CheWNEWFEB0002_full.rec" > <i class="download icon"></i> Download </a></p&gt