UU 2801- P221D delta P1P2

Strain P221D (low kinase activity) with P1P2 deletion in CheA<strong>Tilt Series Date:</strong> 2012-09-17</p> <strong>Data Taken By:</strong> Ariane Briegel</p> <strong>Species / Specimen:</strong> Escherichia coli</p> <strong>Strain:</strong> RP 437</p> <strong>Tilt Series Settings:</strong> Single Axis, tilt range: (-64.0°, 64.0°), step: 1°, constant angular increment, dosage: 160.0 eV/Ų, defocus: -8.0 μm, magnification: 34000x. </p> <strong>Acquisition Software:</strong> UCSFTomo</p> <strong>Upload Method:</strong> pipeline</p> <strong>Processing Software Used:</strong> UCSFtomo, IMOD, tomo3D</p> <strong>Collaborators and Roles:</strong> John S. Parkinson</p> <strong>Purification / Growth Conditions / Treatment:</strong> cells grown to log phase, then lysed for one hour with Penicillin</p> <strong>Sample Preparation:</strong> cells plunge frozen on Quantifoul R2/2 in liquid ethane/propane</p>Files available via S3 at https://renc.osn.xsede.org/ini210004tommorrell/tomography_archive/aba2012-09-17-6</p>Coli2801atlas0917_10002.mrc, Tilt Series (Pixel Size 0.33 nm), 3.7 GB <a role="button" class="ui compact mini button" href="https://renc.osn.xsede.org/ini210004tommorrell/tomography_archive/aba2012-09-17-6/rawdata/Coli2801atlas0917_10002.mrc" > <i class="download icon"></i> Download </a></p> Coli2801atlas0917_10002_part130_3.rec, Reconstruction (Pixel Size 0.66 nm), 2.8 GB <a role="button" class="ui compact mini button" href="https://renc.osn.xsede.org/ini210004tommorrell/tomography_archive/aba2012-09-17-6/3dimage_20818/Coli2801atlas0917_10002_part130_3.rec" > <i class="download icon"></i> Download </a></p&gt