Cancer cell invasion and EMT marker expression: A three-dimensional study of the human cancer-host interface
- Bronsert, P.
- Enderle-Ammour, K.
- Bader, M.
- Timme, S.
- Kuehs, M.
- Csanadi, A.
- Kayser, G.
- Kohler, I.
- Bausch, D.
- Hoeppner, J.
- Hopt, U.T.
- Keck, T.
- Stickeler, E.
- Passlick, B.
- Schilling, O.
- Reiss, C.P.
- Vashist, Y.
- Brabletz, T.
- Berger, J.
- Lotz, J.
- Olesch, J.
- Werner, M.
- Wellner, U.F.
DOIAbstract
Cancer cell invasion takes place at the cancer-host interface and is a prerequisite for distant metastasis. The relationships between current biological and clinical concepts such as cell migration modes, tumour budding and epithelial-mesenchymal transition (EMT) remains unclear in several aspects, especially for the 'real' situation in human cancer. We developed a novel method that provides exact three-dimensional (3D) information on both microscopic morphology and gene expression, over a virtually unlimited spatial range, by reconstruction from serial immunostained tissue slices. Quantitative 3D assessment of tumour budding at the cancer-host interface in human pancreatic, colorectal, lung and breast adenocarcinoma suggests collective cel...
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