Concept development for an extended plant test in the environmental risk assessment of veterinary medicinal products: UBA-FB 002048/E

In the frame of this research project, an extended terrestrial plant test for veterinary medicinal products - especially antibiotics - with a more realistic exposure scenario via manure application was developed. This research project comprehends: i) development of methods of preparation, acclimation, incubation, and application of manure in a plant test, ii) tests investigating necessary technical background (e.g. suitable plant species, suitable manure concentration), iii) tests according to the OECD 208 standard test design and modified test designs, considering an application of the test substance via manure with and without incubation. Main tests were conducted with six plant species, pig and cattle manure, and two representative veterinary antibiotics (florfenicol and tylosin tartrate). The test design considered additional effects of manure to the test substance (e.g. adsorption) as well as transformation/metabolization of the test substance in manure and formation of NER. This report describes the results of the tests which were carried out by Fraunhofer IME and ECT Oekotoxikologie GmbH and a manual for an extended plant test with an exposure scenario via manure application after an anaerobic incubation period for the use in environmental risk assessment of veterinary medicinal products is presented