Zirkulationssystem sowie Verfahren zur Vitalversorgung von Zellkulturen in einem mikrofluidischen Netzwerk

Source: WO15003997A1 [EN] The invention relates to a circulation system and a method for vital supply of cell cultures in a microfluidic network which is formed with several cell culture compartments (1), each having at least one input (1.1) and at least one output (1.2). The cell culture compartments (1) are connected to at least one reservoir (2) containing nutrient solution, the connection being effected via a first microchannel structure (3), which has at least one first-order branch (3.1). A delivery device (4) is also present, with which the nutrient solution can be delivered from the reservoir (2) to the cell culture compartments (1) via the microchannel structure (3). According to the invention, a volumetric flow regulator (5), with which a volumetric flow of the nutrient solution can be regulated, is arranged in each arm of the first-order branch (3.1) and/or in the course of each microchannel opening directly into an input (1.1) of a cell culture compartment (1)