The Levels of a Universally Conserved tRNA Modification Regulate Cell Growth

Artículo de publicación ISIN-6-Threonylcarbamoyl-adenosine (t(6)A) is a universal modification occurring at position 37 in nearly all tRNAs that decode A-starting codons, including the eukaryotic initiator tRNA (tRNA(i)(Met)). Yeast lacking central components of the t(6)A synthesis machinery, such as Tcs3p (Kae1p) or Tcs5p (Bud32p), show slow-growth phenotypes. In the present work, we show that loss of the Drosophila tcs3 homolog also leads to a severe reduction in size and demonstrate, for the first time in a non-microbe, that Tcs3 is required for t(6)A synthesis. In Drosophila and in mammals, tRNA(i)(Met) is a limiting factor for cell and animal growth. We report that the t(6)A-modified form of tRNA(i)(Met) is the actual limiting factor. We show that changing the proportion of t(6)A-modified tRNA(i)(Met), by expression of an un-modifiable tRNA(i)(Met) or changing the levels of Tcs3, regulate target of rapamycin (TOR) kinase activity and influences cell and animal growth in vivo. These findings reveal an unprecedented relationship between the translation machinery and TOR, where translation efficiency, limited by the availability of t(6)A-modified tRNA, determines growth potential in eukaryotic cells.Company of Biologists International Union of Biochemistry and Molecular Biology National Institutes of Health P40OD01853