Escherichia coli phosphofructokinase-2 (Pfk-2) is a homodimer whose subunits consist of a large domain and an additional b-sheet that provides the interfacial contacts between the subunits, creating a b-barrel flattened-like structure with the adjacent subunit’s b-sheet. To determine how the structural organization of Pfk-2 determines its stability, the reversible unfolding of the enzyme was characterized under equilibrium conditions by enzymatic activity, circular dichroism, fluorescence and hydrodynamic measurements. Pfk-2 undergoes a cooperative unfolding/dissociation process with the accumulation of an expanded and unstructured monomeric intermediate with a marginal stability and a large solvent accessibility with respect to the ...
In a previous work, chemical modification of Cys-238 of Escherichia coli Pfk-2 raised concerns on th...
The aggregation states of Escherichia coli phosphofructokinase 2 (Pfk-2) and of a mutant enzyme (Pfk...
Phosphofructokinase-1 and -2 (Pfk-1 and Pfk-2, respectively) from Escherichia coli belong to differ...
Escherichia coli phosphofructokinase-2 (Pfk-2) is a homodimer whose subunits consist of a large dom...
AbstractEscherichia coli phosphofructokinase-2 (Pfk-2) is a homodimer whose subunits consist of a la...
Publicación ISIEscherichia coli phosphofructokinase-2 (Pfk-2) is an oligomeric enzyme characterized ...
Phosphofructokinase-2 is a 66 kD homodimer whose subunits are associated by means of a bimolecular d...
AbstractPhosphofructokinase-2 is a 66kD homodimer whose subunits are associated by means of a bimole...
AbstractEscherichia coli phosphofructokinase-2 (Pfk-2) is an obligate homodimer that follows a highl...
Escherichia coli phosphofructokinase-2 (Pfk-2) is an obligate homodimer that follows a highly cooper...
© 2015 Biophysical Society. Escherichia coli phosphofructokinase-2 (Pfk-2) is an obligate homodimer ...
AbstractFolding studies have been focused mainly on small, single-domain proteins or isolated single...
Folding studies have been focused mainly on small, single-domain proteins or isolated single domains...
Modification of Escherichia coli phosphofructokinase-2 (Pfk-2) with N- (1-pyrenil)maleimide results ...
AbstractIn a previous work, chemical modification of Cys-238 of Escherichia coli Pfk-2 raised concer...
In a previous work, chemical modification of Cys-238 of Escherichia coli Pfk-2 raised concerns on th...
The aggregation states of Escherichia coli phosphofructokinase 2 (Pfk-2) and of a mutant enzyme (Pfk...
Phosphofructokinase-1 and -2 (Pfk-1 and Pfk-2, respectively) from Escherichia coli belong to differ...
Escherichia coli phosphofructokinase-2 (Pfk-2) is a homodimer whose subunits consist of a large dom...
AbstractEscherichia coli phosphofructokinase-2 (Pfk-2) is a homodimer whose subunits consist of a la...
Publicación ISIEscherichia coli phosphofructokinase-2 (Pfk-2) is an oligomeric enzyme characterized ...
Phosphofructokinase-2 is a 66 kD homodimer whose subunits are associated by means of a bimolecular d...
AbstractPhosphofructokinase-2 is a 66kD homodimer whose subunits are associated by means of a bimole...
AbstractEscherichia coli phosphofructokinase-2 (Pfk-2) is an obligate homodimer that follows a highl...
Escherichia coli phosphofructokinase-2 (Pfk-2) is an obligate homodimer that follows a highly cooper...
© 2015 Biophysical Society. Escherichia coli phosphofructokinase-2 (Pfk-2) is an obligate homodimer ...
AbstractFolding studies have been focused mainly on small, single-domain proteins or isolated single...
Folding studies have been focused mainly on small, single-domain proteins or isolated single domains...
Modification of Escherichia coli phosphofructokinase-2 (Pfk-2) with N- (1-pyrenil)maleimide results ...
AbstractIn a previous work, chemical modification of Cys-238 of Escherichia coli Pfk-2 raised concer...
In a previous work, chemical modification of Cys-238 of Escherichia coli Pfk-2 raised concerns on th...
The aggregation states of Escherichia coli phosphofructokinase 2 (Pfk-2) and of a mutant enzyme (Pfk...
Phosphofructokinase-1 and -2 (Pfk-1 and Pfk-2, respectively) from Escherichia coli belong to differ...