Increased β-oxidation with improved glucose uptake capacity in adipose tissue from obese after weight loss and maintenance.

OBJECTIVE: We investigated protein markers for pathways of the fatty glucose metabolism in human adipose tissue after a weight loss program restriction. DESIGN AND METHODS: Overweight/obese subjects underwent an intervention of 5 weeks of a very low calorie diet followed by a 3 weeks maintenance diet. Abdominal subcutaneous adipose tissue biopsies were before and after the intervention. 17 target proteins as markers of pathways for the uptake and handling of fatty acids and glucose, were by Western blotting and 11 were retrieved from previous proteomics work. Correlation coefficients were calculated among changes of these RESULTS: 3-hydroxyacyl-CoA dehydrogenase (HADH), catalase, fatty acid translocase, fatty acid transporter protein 3, adipose triglyceride acid binding protein 4, aldolase-C, tubulin-beta-5, and annexin A2 significantly, and lipoprotein lipase, perilipin 1 and hormone-sensitive tended to change. On average increased GLUT4-translocation was observed, supported by a consistent increase of tyr-24 phosphorylated annexin A2. CONCLUSIONS: Our findings suggest that after weight loss by calorie and a short-period of maintenance, adipose tissue has an increased glucose uptake, and lipid mobilization and oxidation. Such metabolic relate to the health benefit of weight loss