Sorghum is an important cereal crop cultivated by smallholder farmers of Mali, contributing significantly to their food demand and security. The study evaluated different fertilization strategies that combined organic and inorganic fertilizer applications with three sorghum varieties. The experiments were conducted over three cropping seasons (2017–2019) in three sites (Bamako, Bougouni, and Koutiala respectively) within the Sudanian region of Mali. Our results showed a significant effect of season, variety, and fertilization strategies on grain and stalk yields. Grain yield increased by 8–40% in Koutiala, 11–53% in Bougouni, and 44–110% in Bamako while the average stalk yield was above 5000 kg h