Construction and application of site-specific artificial nucleases for targeted gene editing
- Kok, Fatma O.
- Gupta, Ankit
- Lawson, Nathan D.
- Wolfe, Scot A.
Publication date
January 2014
DOI Publisher
Springer Science and Business Media LLC ISSN
1064-3745 Citation count (estimate)
6Abstract
Artificial nucleases have developed into powerful tools for introducing precise genome modifications in a wide variety of species. In this chapter the authors provide detailed protocols for rapidly constructing zinc finger nucleases (ZFNs) and TALE nucleases (TALENs) and evaluating their activity for the targeted generation of InDels within the zebrafish genome
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