Yeast one-hybrid (Y1H) assays provide a gene-centered method for the identification of interactions between gene promoters and regulatory transcription factors (TFs). To date, Y1H assays have involved library screens that are relatively expensive and laborious. We present two Y1H strategies that allow immediate prey identification: matrix assays that use an array of 755 individual Caenorhabditis elegans TFs, and smart-pool assays that use TF multiplexing. Both strategies simplify the Y1H pipeline and reduce the cost of protein-DNA interaction identification. We used a Steiner triple system (STS) to create smart pools of 4-25 TFs. Notably, we uniplexed a small number of highly connected TFs to allow efficient assay deconvolution. Both strate...
Since its development about two decades ago, the yeast one-hybrid (Y1H) assay has become an importan...
Since their original description more than 25 years ago, the yeast one- and two-hybrid systems (Y1H/...
Since their original description more than 25 years ago, the yeast one- and two-hybrid systems (Y1H/...
Yeast one- hybrid ( Y1H) assays provide a gene- centered method for the identification of interactio...
Yeast one- hybrid ( Y1H) assays provide a gene- centered method for the identification of interactio...
Yeast one- hybrid ( Y1H) assays provide a gene- centered method for the identification of interactio...
A major challenge in systems biology is to understand the gene regulatory networks that drive develo...
This protocol describes how to perform a gateway-compatible yeast one-hybrid screen. Protein-DNA in...
An important question when studying gene regulation is which transcription factors (TFs) interact wi...
Gateway-compatible yeast one-hybrid (Y1H) assays provide a convenient gene-centered (DNA to protein)...
Yeast one-hybrid (Y1H) assays are used to identify which transcription factor (TF) prey molecules ...
Identifying the sets of transcription factors (TFs) that regulate each human gene is a daunting task...
Since the advent of microarrays, vast amounts of gene expression data have been generated. However, ...
Yeast one-hybrid (Y1H) assays are used to identify which transcription factor (TF) preys can bind ...
In recent years, new techniques have spurred the discovery of cis-regulatory DNA elements. These str...
Since its development about two decades ago, the yeast one-hybrid (Y1H) assay has become an importan...
Since their original description more than 25 years ago, the yeast one- and two-hybrid systems (Y1H/...
Since their original description more than 25 years ago, the yeast one- and two-hybrid systems (Y1H/...
Yeast one- hybrid ( Y1H) assays provide a gene- centered method for the identification of interactio...
Yeast one- hybrid ( Y1H) assays provide a gene- centered method for the identification of interactio...
Yeast one- hybrid ( Y1H) assays provide a gene- centered method for the identification of interactio...
A major challenge in systems biology is to understand the gene regulatory networks that drive develo...
This protocol describes how to perform a gateway-compatible yeast one-hybrid screen. Protein-DNA in...
An important question when studying gene regulation is which transcription factors (TFs) interact wi...
Gateway-compatible yeast one-hybrid (Y1H) assays provide a convenient gene-centered (DNA to protein)...
Yeast one-hybrid (Y1H) assays are used to identify which transcription factor (TF) prey molecules ...
Identifying the sets of transcription factors (TFs) that regulate each human gene is a daunting task...
Since the advent of microarrays, vast amounts of gene expression data have been generated. However, ...
Yeast one-hybrid (Y1H) assays are used to identify which transcription factor (TF) preys can bind ...
In recent years, new techniques have spurred the discovery of cis-regulatory DNA elements. These str...
Since its development about two decades ago, the yeast one-hybrid (Y1H) assay has become an importan...
Since their original description more than 25 years ago, the yeast one- and two-hybrid systems (Y1H/...
Since their original description more than 25 years ago, the yeast one- and two-hybrid systems (Y1H/...