Add to ORKGThe suppression patterns of 11 phage P22 mutants bearing different amber mutations in the gene encoding lysozyme (19) were determined on six different amber suppressor strains. Of the 60 resulting single amino acid substitutions, 18 resulted in defects in lysozyme activity at 30 degrees; an additional seven were defective at 40 degrees. Revertants were isolated on the missuppressing hosts following UV mutagenesis; they were screened to distinguish primary- from second-site revertants. It was found that second-site revertants were recovered with greater efficiency if the UV-irradiated phage stocks were passaged through an intermediate host in liquid culture rather than plated directly on the nonpermissive host. Eleven second-site revertant...
maps in the CP repressor gene, and VX, which maps between cz and cQ. Although neither P22 K5 nor P22...
Phage mutants were isolated with amber mutations in genes necessary for establishment of lysogeny. T...
Phage P22 induces a lysozyme in Salmonella typhimurium cells toward the later stage of its multiplic...
P22 lysozyme, encoded by gene 19, is an essential phage protein responsible for hydrolyzing the bact...
A P22 hybrid phage bearing the bacteriophage T4 lysozyme gene (e), as well as T4 sequences upstream ...
Amber mutations were introduced into every codon (except the initiating AUG) of the bacteriophage T4...
A plasmid-borne gene encoding bacteriophage T4 lysozyme with a structural mutation, Tyr161-Ala, was ...
A plasmid based genetic system was developed for the tail protein of the Salmonella typhimurium bact...
A plasmid based genetic system was developed for the tail protein of the Salmonella typhimurium bact...
Two temperature-sensitive mutants of phage P22, ts18.1 and ts12.1, are described which have recessiv...
The amino acid sequence of the lysozyme from phage P22 is shown to be homologous (26% identity) with...
Model-free approaches (random mutagenesis, DNA shuffling) in combination with more rational, three...
Bacteriophage genomes encode lysozymes whose role is to favour the release of virions by lysis of th...
Two temperature-sensitive mutants of phage P22, ts18.1 and ts12.1, are described which have recessiv...
Wild-type and amber mutant alleles of the lysis genes of P22 were cloned and sequenced. Gene 13 enco...
maps in the CP repressor gene, and VX, which maps between cz and cQ. Although neither P22 K5 nor P22...
Phage mutants were isolated with amber mutations in genes necessary for establishment of lysogeny. T...
Phage P22 induces a lysozyme in Salmonella typhimurium cells toward the later stage of its multiplic...
P22 lysozyme, encoded by gene 19, is an essential phage protein responsible for hydrolyzing the bact...
A P22 hybrid phage bearing the bacteriophage T4 lysozyme gene (e), as well as T4 sequences upstream ...
Amber mutations were introduced into every codon (except the initiating AUG) of the bacteriophage T4...
A plasmid-borne gene encoding bacteriophage T4 lysozyme with a structural mutation, Tyr161-Ala, was ...
A plasmid based genetic system was developed for the tail protein of the Salmonella typhimurium bact...
A plasmid based genetic system was developed for the tail protein of the Salmonella typhimurium bact...
Two temperature-sensitive mutants of phage P22, ts18.1 and ts12.1, are described which have recessiv...
The amino acid sequence of the lysozyme from phage P22 is shown to be homologous (26% identity) with...
Model-free approaches (random mutagenesis, DNA shuffling) in combination with more rational, three...
Bacteriophage genomes encode lysozymes whose role is to favour the release of virions by lysis of th...
Two temperature-sensitive mutants of phage P22, ts18.1 and ts12.1, are described which have recessiv...
Wild-type and amber mutant alleles of the lysis genes of P22 were cloned and sequenced. Gene 13 enco...
maps in the CP repressor gene, and VX, which maps between cz and cQ. Although neither P22 K5 nor P22...
Phage mutants were isolated with amber mutations in genes necessary for establishment of lysogeny. T...
Phage P22 induces a lysozyme in Salmonella typhimurium cells toward the later stage of its multiplic...