The blood-brain barrier (BBB) represents an obstacle in targeting and delivering therapeutics to the central nervous system. In order to discover new BBB-targeting molecules, we panned a phage-displayed nonimmune human single-chain antibody fragment (scFv) library against a representative BBB model comprised of hydrocortisone-treated primary rat brain endothelial cells. Parallel screens were performed with or without pre-subtraction against primary rat heart and lung endothelial cells in an effort to identify antibodies that may have binding selectivity toward brain endothelial cells. After three rounds of screening, three unique scFvs, scFv15, scFv38, and scFv29, were identified that maintained binding to primary rat brain endothelial cell...
The blood\u2010brain barrier (BBB) is a formidable obstacle to the delivery of therapeutics to the b...
The blood-brain barrier (BBB) blocks molecules larger than about 500 Daltons from escaping the brain...
The identification of marker molecules specific for blood and lymphatic endothelium may provide new ...
A major bottleneck in the successful development of central nervous system (CNS) drugs is the discov...
Antibodies against receptors that undergo transcytosis across the blood\u2013brain barrier (BBB) hav...
PurposeTransport of drugs to the brain is limited by the blood-brain barrier. New, specific brain en...
Antibodies against various receptors that undergo transcytosis across brain capillary endothelium in...
The blood brain barrier (BBB) is often regarded as a passive barrier that protects brain parenchyma ...
Barrier vessels in the central nervous system are lined with endothelial cells which constitute the ...
FC5 and FC44 are single-domain antibodies (VHHs), selected by functional panning of phage-display ll...
Abstract The blood–brain barrier (BBB) is a highly specialized neurovascular unit, initially describ...
Blood-brain barrier (BBB) characteristics are induced and maintained by cross-talk between brain mic...
While the concept of the <em>Neurovascular Unit</em> (NVU) is increasingly recognized for exploring ...
The endothelial barrier antigen (EBA) is a membrane protein expressed by endothelial cells of the ra...
Current methods for examining antibody trafficking are either non-quantitative such as immunocytoche...
The blood\u2010brain barrier (BBB) is a formidable obstacle to the delivery of therapeutics to the b...
The blood-brain barrier (BBB) blocks molecules larger than about 500 Daltons from escaping the brain...
The identification of marker molecules specific for blood and lymphatic endothelium may provide new ...
A major bottleneck in the successful development of central nervous system (CNS) drugs is the discov...
Antibodies against receptors that undergo transcytosis across the blood\u2013brain barrier (BBB) hav...
PurposeTransport of drugs to the brain is limited by the blood-brain barrier. New, specific brain en...
Antibodies against various receptors that undergo transcytosis across brain capillary endothelium in...
The blood brain barrier (BBB) is often regarded as a passive barrier that protects brain parenchyma ...
Barrier vessels in the central nervous system are lined with endothelial cells which constitute the ...
FC5 and FC44 are single-domain antibodies (VHHs), selected by functional panning of phage-display ll...
Abstract The blood–brain barrier (BBB) is a highly specialized neurovascular unit, initially describ...
Blood-brain barrier (BBB) characteristics are induced and maintained by cross-talk between brain mic...
While the concept of the <em>Neurovascular Unit</em> (NVU) is increasingly recognized for exploring ...
The endothelial barrier antigen (EBA) is a membrane protein expressed by endothelial cells of the ra...
Current methods for examining antibody trafficking are either non-quantitative such as immunocytoche...
The blood\u2010brain barrier (BBB) is a formidable obstacle to the delivery of therapeutics to the b...
The blood-brain barrier (BBB) blocks molecules larger than about 500 Daltons from escaping the brain...
The identification of marker molecules specific for blood and lymphatic endothelium may provide new ...