This study presents the development of ultra-performance liquid chromatography (UPLC) mass spectrometry (MS) combined with electrochemistry (EC) for the first time and its application for the structural analysis of proteins/peptides that contain disulfide bonds. In our approach, a protein/peptide mixture sample undergoes a fast UPLC separation and subsequent electrochemical reduction in an electrochemical flow cell followed by online MS and tandem mass spectrometry (MS/MS) analyses. The electrochemical cell is coupled to the mass spectrometer using our recently developed desorption electrospray ionization (DESI) interface. Using this UPLC/EC/DESI-MS method, peptides that contain disulfide bonds can be differentiated from those without disul...
Electrospray ionisation (ESI) coupled to mass spectrometry (MS) is one of the most important detecti...
Electrochemical protein digestion prior to mass spectrometric analysis is a purely instrumental appr...
Enhanced charging, or supercharging, of analytes in electrospray ionization mass spectrometry (ESI M...
Chemical cross-linking combined with mass spectrometry (MS) is powerful to provide protein three-dim...
Abstract. Particularly in the field of middle- and top-down peptide and protein analysis, disulfide ...
Abstract. Particularly in the field of middle- and top-down peptide and protein analysis, disulfide ...
The disulfide bond is an important post-translational modification to form and maintain the native s...
Structural confirmation and quality control of recombinant monoclonal antibodies (mAbs) by top-down ...
The characterization of mechanisms, analytical benefits, and applications of two different methods f...
Characterization of disulfide bond-containing proteins by hydrogen/deuterium exchange monitored by m...
Disulfide bonds play important roles in establishing and stabilizing three-dimensional protein struc...
Electron-based tandem mass spectrometry (MS/MS) techniques, e.g., electron capture dissociation (ECD...
Electrochemical protein digestion prior to mass spectrometric analysis is a purely instrumental appr...
Electrospray ionisation (ESI) coupled to mass spectrometry (MS) is one of the most important detecti...
We have previously shown that liquid sample desorption electrospray ionization-mass spectrometry (DE...
Electrospray ionisation (ESI) coupled to mass spectrometry (MS) is one of the most important detecti...
Electrochemical protein digestion prior to mass spectrometric analysis is a purely instrumental appr...
Enhanced charging, or supercharging, of analytes in electrospray ionization mass spectrometry (ESI M...
Chemical cross-linking combined with mass spectrometry (MS) is powerful to provide protein three-dim...
Abstract. Particularly in the field of middle- and top-down peptide and protein analysis, disulfide ...
Abstract. Particularly in the field of middle- and top-down peptide and protein analysis, disulfide ...
The disulfide bond is an important post-translational modification to form and maintain the native s...
Structural confirmation and quality control of recombinant monoclonal antibodies (mAbs) by top-down ...
The characterization of mechanisms, analytical benefits, and applications of two different methods f...
Characterization of disulfide bond-containing proteins by hydrogen/deuterium exchange monitored by m...
Disulfide bonds play important roles in establishing and stabilizing three-dimensional protein struc...
Electron-based tandem mass spectrometry (MS/MS) techniques, e.g., electron capture dissociation (ECD...
Electrochemical protein digestion prior to mass spectrometric analysis is a purely instrumental appr...
Electrospray ionisation (ESI) coupled to mass spectrometry (MS) is one of the most important detecti...
We have previously shown that liquid sample desorption electrospray ionization-mass spectrometry (DE...
Electrospray ionisation (ESI) coupled to mass spectrometry (MS) is one of the most important detecti...
Electrochemical protein digestion prior to mass spectrometric analysis is a purely instrumental appr...
Enhanced charging, or supercharging, of analytes in electrospray ionization mass spectrometry (ESI M...