Add to ORKGA method for selecting amino acid residues is disclosed which upon replacement will give rise to an enzyme with an altered pH optimum. The method is specific for metalloenzymes which are inactivated at low pH due to the dissociation of the metal ions. The method is based on altering the pKa of the metal coordinating ligands or altering the Kassfor the metal binding. New glucose isomerases with an altered pH optimum are provided according to this method. These altered properties enable starch degradation to be performed at lower pH values.</p
The industrial processes often require enzymes to function under very specific reaction condition. E...
Directed evolution of secreted industrial enzymes is hampered by the lack of powerful selection tech...
Procedures are described for linking monomethoxypoly(ethylene glycol) (mPEG) to both epsilon and alp...
A method for selecting amino acid residues is disclosed which upon replacement will give rise to an ...
Human glucose phosphate isomerase was subjected to a series of chemical modifications aimed at ident...
optimum from Thermoanaerobacterium strain xylose (glucose) isomerase with an acidic pH Purification ...
This invention relates to a process for the production of new mutant glucose isomerases which have i...
A low-calorie sugar-substituting sweetener, d-tagatose, can be produced by l-arabinose isomerase (l-...
Glucoamylase (EC 3.2.1.3) (GA), an enzyme that catalyzes the release of [beta]-D-glucose from the no...
Structural properties of phosphoglucose isomerases isolated from a variety of species have been comp...
【Purpose】D-Tagatose is a naturally occurring ketohexose considered to be a potential reduced-energy ...
The objective of this study is to produce and purify glucose isomerase (GI) from Bacillus megaterium...
In a previous communication it has been shown by Basu and Nandi that the extent of inactivation cons...
Background: L-arabinose isomerases catalyse the isomerization of L-arabinose into L-ribulose at insi...
The native sago starch exists as a compact crystalline structure and is not efficiently hydrolyzed b...
The industrial processes often require enzymes to function under very specific reaction condition. E...
Directed evolution of secreted industrial enzymes is hampered by the lack of powerful selection tech...
Procedures are described for linking monomethoxypoly(ethylene glycol) (mPEG) to both epsilon and alp...
A method for selecting amino acid residues is disclosed which upon replacement will give rise to an ...
Human glucose phosphate isomerase was subjected to a series of chemical modifications aimed at ident...
optimum from Thermoanaerobacterium strain xylose (glucose) isomerase with an acidic pH Purification ...
This invention relates to a process for the production of new mutant glucose isomerases which have i...
A low-calorie sugar-substituting sweetener, d-tagatose, can be produced by l-arabinose isomerase (l-...
Glucoamylase (EC 3.2.1.3) (GA), an enzyme that catalyzes the release of [beta]-D-glucose from the no...
Structural properties of phosphoglucose isomerases isolated from a variety of species have been comp...
【Purpose】D-Tagatose is a naturally occurring ketohexose considered to be a potential reduced-energy ...
The objective of this study is to produce and purify glucose isomerase (GI) from Bacillus megaterium...
In a previous communication it has been shown by Basu and Nandi that the extent of inactivation cons...
Background: L-arabinose isomerases catalyse the isomerization of L-arabinose into L-ribulose at insi...
The native sago starch exists as a compact crystalline structure and is not efficiently hydrolyzed b...
The industrial processes often require enzymes to function under very specific reaction condition. E...
Directed evolution of secreted industrial enzymes is hampered by the lack of powerful selection tech...
Procedures are described for linking monomethoxypoly(ethylene glycol) (mPEG) to both epsilon and alp...