Add to ORKGThe invention relates to an isolated polynucleotide sequence comprising a nucleic acid sequence encoding the amino acid sequence of KshA protein or of KshB protein, encoded by nucleotides 499-1695 of SEQ ID NO: 1 or by nucleotides 387-1427 of SEQ ID NO:2, respectively, and functional homologues thereof. The polynucleotides of the invention can be used to construct genetically modified microorganisms blocked in 3-ketosteroid 9alpha-hydroxylase activity, which are useful in the microbial degradation of steroids to accumulate certain steroid products.</p
The biochemistry and genetics of the bacterial steroid catabolism have been extensively studied duri...
The Arthrobacter simplex gene coding for 3-ketosteroid-Δ1-dehydrogenase, a key enzyme in the degrada...
3-Ketosteroid Delta(1)-dehydrogenases are FAD-dependent enzymes that catalyze the introduction of a ...
The invention relates to an isolated polynucleotide sequence comprising a nucleic acid sequence enco...
A method is described to construct genetically modified strains of steroid degrading micro-organisms...
Various micro-organisms are able to use sterols/steroids as carbon- and energy sources for growth. 3...
The well-known large catabolic potential of rhodococci is greatly facilitated by an impressive gene ...
A method is described to construct genetically modified strains of steroid degrading micro-organisms...
Various micro-organisms are able to use sterols/steroids as carbon- and energy sources for growth. 3...
This paper reports the biochemical characterization of a purified and reconstituted two-component 3-...
Previously we have characterized 3-ketosteroid 9 alpha-hydroxylase (KSH), a key enzyme in microbial ...
The biochemistry and genetics of the bacterial steroid catabolism have been extensively studied duri...
The Arthrobacter simplex gene coding for 3-ketosteroid-Δ1-dehydrogenase, a key enzyme in the degrada...
3-Ketosteroid Delta(1)-dehydrogenases are FAD-dependent enzymes that catalyze the introduction of a ...
The invention relates to an isolated polynucleotide sequence comprising a nucleic acid sequence enco...
A method is described to construct genetically modified strains of steroid degrading micro-organisms...
Various micro-organisms are able to use sterols/steroids as carbon- and energy sources for growth. 3...
The well-known large catabolic potential of rhodococci is greatly facilitated by an impressive gene ...
A method is described to construct genetically modified strains of steroid degrading micro-organisms...
Various micro-organisms are able to use sterols/steroids as carbon- and energy sources for growth. 3...
This paper reports the biochemical characterization of a purified and reconstituted two-component 3-...
Previously we have characterized 3-ketosteroid 9 alpha-hydroxylase (KSH), a key enzyme in microbial ...
The biochemistry and genetics of the bacterial steroid catabolism have been extensively studied duri...
The Arthrobacter simplex gene coding for 3-ketosteroid-Δ1-dehydrogenase, a key enzyme in the degrada...
3-Ketosteroid Delta(1)-dehydrogenases are FAD-dependent enzymes that catalyze the introduction of a ...