Low-methoxyl lemon pectin attenuates inflammatory responses and improves intestinal barrier integrity in caerulein-induced experimental acute pancreatitis

Scope: Acute pancreatitis (AP) is a common clinical acute abdominal disease. The intestinal injury associated with AP will aggravate the condition retroactively. This study investigates whether the low-methoxyl pectin (LMP) isolated from lemon could attenuate AP and associated intestinal injury.Methods and results: Experimental AP was induced in BALB/c mice by caerulien (CAE) hyperstimulation. Nutritional prophylactic group was pre-fed with 5% LMP supplemented forage 3 days before AP induction. We found that LMP supplementation attenuated the severity of AP as evidenced by reduced serum amylase and lipase levels, pancreatic edema and myeloperoxidase activity. The protective effect was also confirmed by histological examination of pancreatic damage. LMP suppressed the production of pancreatic proinflammatory cytokines including TNF-alpha, IL-1 beta, and IL-6. Moreover, LMP supplementation restored AP-associated disruption of intestinal barrier integrity as evidenced by upregulation of tight junction modulatory proteins occludin, zonula occludens (ZO)-1, antimicrobial peptides beta-defensin-1 (DEFB1) and CRAMP as well as increase in SCFAs production. LMP supplemented mice with AP exhibited suppressed intestinal inflammation as shown by decreased ileal and colon cytokine production compared with CAE group.Conclusion: Our results support dietary LMP supplementation as an effective nutritional intervention for AP and associated intestinal injury.