Comparison of three methods for competitive binding of monoclonal antibodies - The localization of antigenic sites for monoclonal antibodies on Panulirus interruptus hemocyanin

The competitive binding of a panel of monoclonal antibodies against hemocyanin of Panulirus interruptus hemocyanin was investigated with three different methods, A competitive-binding immunoassay method was more successful in the determination of ternary complexes than gel electrophoresis and gel filtration. The latter two methods could only be applied with antibodies possessing a higher affinity, Eleven monoclonal antibodies were assigned to groups on the basis of their interactions with five antigenic regions.