pSEUDO, a Genetic Integration Standard for Lactococcus lactis
- Pinto, Joao P. C.
- Zeyniyev, Araz
- Karsens, Harma
- Trip, Hein
- Lolkema, Juke S.
- Kuipers, Oscar P.
- Kok, Jan
DOIAbstract
Plasmid pSEUDO and derivatives were used to show that llmg_pseudo_10 in Lactococcus lactis MG1363 and its homologous locus in L. lactis IL1403 are suitable for chromosomal integrations. L. lactis MG1363 and IL1403 nisin-induced controlled expression (NICE) system derivatives (JP9000 and IL9000) and two general stress reporter strains (NZ9000::PhrcA-GFP and NZ9000::PgroES-GFP) enabling in vivo noninvasive monitoring of cellular fitness were constructed.</p
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