Recovery of type I fiber regionalization in gastrocnemius medialis of the rat after reinnervation along original and foreign paths, with and without muscle rotation

After reinnervation following transection of the sciatic nerve, normal patterns of regional type I fiber distribution are known to return in rat hindlimb muscles. Here we investigate how this recovery is influenced by experimental conditions. In an initial operation, the nerve of gastrocnemius medialis (GM) of adult rats was cut close to the muscle and reinserted either (i) close to the original nerve entry, or (ii) at a more medial 'foreign' site. In other groups of animals, these nerve operations were combined with a rotation of the GM muscle around its longitudinal axis, trying to ascertain whether the position of the muscle within the limb was of importance for the reinnervation processes. In a control group the muscle was rotated but innervation remained intact. After 21 weeks, the GM muscles were removed from both hindlimbs. Cross-sections were cut at seven different levels along each muscle, and 'slow' type I fibers were identified after staining for myofibrillar ATPase. The topographical positions were mapped out for all type I fibers. In all reinnervated muscles, an extensive type I fiber grouping was seen, indicating a widespread respecification of muscle fiber properties by ingrowing 'slow' axons. Normal topographical directions of type I fiber regionalization were about equally well restored in groups with the nerve inserted at the original or at the foreign site. In rotated muscles, the direction of type I fiber regionalization was significantly less rotated than the muscle as a whole. The results suggest that ingrowing 'slow' motor axons are guided toward their normal 'slow' regions by clues which are largely independent of the i.m. path of regeneration (original vs. foreign nerve entry site) but partly dependent on the position of the target muscle within the limb (rotated vs. non-rotated cases). (C) 2002 IBRO. Published by Elsevier Science Ltd. All rights reserved.