ANTIBIOFILM POTENTIAL OF ENZYMES AS ECOFRIENDLY ANTIFOULING ADDITIVES

Marine biofilms form is the first step of fouling process and after film layer has formed, macro organisms hold on smoothly over this film and mature the fouling process. Every year billions of dollars have been spent in order to prevent biofouling situation. Whereas heavy metals and biocide have been used more in antifouling coating today studies about improving eco-friendly nontoxic coating have been becoming widespread. For this purpose, in our study, different concentration of varied commercially available hydrolytic enzymes and enzymes mix (Protease, alpha-Amilase, Lipase, Xylanase, Pectinase, Glucanase, Viscozyme, and Lysing Complex) were experimented as environment friendly antifouling agent over the biofilm bacteria Pseudoalteromonas porphyrae FJ200650 and Pseudoalteromonas elyakovii FJ200649 which were isolated from Izmir Bay. The antibiofilm potential of enzymes was evaluated separately using the fluorescence microplate reader at varying concentrations. The experimental method was based on total biofilm biomass quantification by the fluorescent dye DAPI after 3 and 24 h incubation period. These analyses were performed as prevention and detachment test. As a result of analysis, it is formalized by the adhesion of marine biofilm-forming bacteria was examined in the effectiveness of hydro-lases in sea-water throughout at 3 and 24 hours' incubation processes. Overall, the mixtures of enzymes can be use as eco-friendly antifouling additives in antifouling coatings