Anti-Cancer Potential of Curcumin in Anaplastic Thyroid Cancer

BACKGROUND AND AIM: Anaplastic Thyroid Cancer (ATK);It is one of the aggressive malignancies whose potential molecularmechanisms have not been elucidated and is the 9th among common cancers. It is resistant to existing cytostatic agents.To supportthe current therapy, the antimetastatic property of Tetrahydrocurcumin (THC) was compared with DX and in-vitro studies wereconducted to demonstrate synergism in combination use.MATERIALS and METHODS: IC25 and IC50 concentrations ofTHC and DX in CAL62 and 8505C cell lines were determined bythe modified MTT method we developed. Cell motility, indicative of metastatic potential; In-vitro wound healing method and 3Dcolony formation potential were evaluated with Agarose Gel Spheroid Model. The effects of treatments on oxidation on cells wereevaluated by TAS and TOS analyzes. SPSS program was used inthe evaluation. Compliance with normal distribution (Kolmogorov-Smirnov/Shapiro-Wilk tests), comparison of groups was evaluated with ANOVA, Significance, Holm-Sidak test.RESULTS: In migration analysis; It was observed that the THC50dose decreased the migration significantly compared to the DX50and DX+THC (Combined) groups (P<0.05). DX+THC; It was observed that it reduced spheroid formation the most, and there was aclose effect between this dose effect and the THC25 dose in termsof spheroid formation and numbers (P<0.05). There was no statistically significant difference in Total Oxidant level between thecombination and control.CONCLUSION: We predict that THC may be effective in metastasis and tumor growth at low doses in ATK cells. We think that incombined use, THC reduces the oxidative stress of DX.Keywords: oxidative stress, antineoplastic, Migration