International audienceMicrotubule self-repair has been studied both in vitro and in vivo as an underlying mechanism of microtubule stability. The turnover of tubulin dimers along the microtubule has challenged the pre-existing dogma that only growing ends are dynamic. However, although there is clear evidence of tubulin incorporation into the shaft of polymerized microtubules in vitro, the possibility of such events occurring in living cells remains uncertain. In this study, we investigated this possibility by microinjecting purified tubulin dimers labeled with a red fluorophore into the cytoplasm of cells expressing GFP-tubulin. We observed the appearance of red dots along the pre-existing green microtubule within minutes. We found that th...
ABSTRACT The dynamics of microtubules in living cells can be seen by fluorescence microscopy when fl...
International audienceMicrotubule instability stems from the low energy of tubulin dimer interaction...
International audienceMicrotubule instability stems from the low energy of tubulin dimer interaction...
International audienceMicrotubule self-repair has been studied both in vitro and in vivo as an under...
International audienceMicrotubule self-repair has been studied both in vitro and in vivo as an under...
Microtubule self-repair has been studied both in vitro and in vivo as an underlying mechanism of mic...
Microtubule self-repair has been studied both in vitro and in vivo as an underlying mechanism of mic...
International audienceThe dynamic instability of microtubules is characterized by slow growth phases...
International audienceThe dynamic instability of microtubules is characterized by slow growth phases...
International audienceThe dynamic instability of microtubules is characterized by slow growth phases...
Microtubules are dynamic polymers that are used for intracellular transport and chromosome segregati...
International audienceThe stochastic switching between microtubule growth and shrinkage is a fascina...
Microtubules are dynamic polymers which play important roles in mitosis, cell locomotion, and the de...
International audienceMicrotubules are dynamic polymers, permanently assembling and disassembling, t...
International audienceMicrotubules are dynamic polymers, permanently assembling and disassembling, t...
ABSTRACT The dynamics of microtubules in living cells can be seen by fluorescence microscopy when fl...
International audienceMicrotubule instability stems from the low energy of tubulin dimer interaction...
International audienceMicrotubule instability stems from the low energy of tubulin dimer interaction...
International audienceMicrotubule self-repair has been studied both in vitro and in vivo as an under...
International audienceMicrotubule self-repair has been studied both in vitro and in vivo as an under...
Microtubule self-repair has been studied both in vitro and in vivo as an underlying mechanism of mic...
Microtubule self-repair has been studied both in vitro and in vivo as an underlying mechanism of mic...
International audienceThe dynamic instability of microtubules is characterized by slow growth phases...
International audienceThe dynamic instability of microtubules is characterized by slow growth phases...
International audienceThe dynamic instability of microtubules is characterized by slow growth phases...
Microtubules are dynamic polymers that are used for intracellular transport and chromosome segregati...
International audienceThe stochastic switching between microtubule growth and shrinkage is a fascina...
Microtubules are dynamic polymers which play important roles in mitosis, cell locomotion, and the de...
International audienceMicrotubules are dynamic polymers, permanently assembling and disassembling, t...
International audienceMicrotubules are dynamic polymers, permanently assembling and disassembling, t...
ABSTRACT The dynamics of microtubules in living cells can be seen by fluorescence microscopy when fl...
International audienceMicrotubule instability stems from the low energy of tubulin dimer interaction...
International audienceMicrotubule instability stems from the low energy of tubulin dimer interaction...