Fluorescence imaging-based screen identifies ARF GEF component of early endosomal trafficking

Endocytic vesicle trafficking is crucial for regulating activity and localization of plasma membrane components, but the process Is still poorly genetically defined In plants. Membrane proteins of the PIN-FORMED (PIN) family exhibit polar localization In plant cells and facilitate cellular efflux of the plant hormone auxin, thereby regulating multiple developmental processes [1, 2]. PIN proteins undergo constitutive endocytosis and GNOM ARF GEF-dependent recycling [3-5], and their localization Is under extensive regulation by developmental and environmental cues [6-9]. We designed a fluorescence Imaging-based screen to Identify Arabidopsis thaliana mutants defective in internalization of proteins including PINs from the plasma membrane. We Identified three mutant loci, BFA-visualized endocytic trafficking defective1 (ben1) through ben3that do not efficiently accumulate PIN1-GFP In Intracellular compartments after Inhibition of recycling and secretion by fungal toxin brefeldin A (BFA). Fine mapping revealed that BEN1 encodes an ARF GEF vesicle trafficking regulator from the functionally uncharacterized BIG class. ben 1 mutant has been previously Implicated in pathogen response [10] and shows cell polarity, BFA sensitivity, and growth defects. BEN1 is Involved in endocytosis of plasma membrane proteins and localizes to early endocytic compartments distinct from GNOM-positive endosomes. Our results Identify BEN1 as the ARF GEF mediating early endosomal traffic