Add to ORKGThough synthesized with a cleavable signal peptide and devoid of membrane anchors, the 262-amino-acid-residue Streptomyces K15 DD-transpeptidase/penicillin-binding protein is membrane-bound. Overexpression in Streptomyces lividans resulted in the export of an appreciable amount of the synthesized protein (4 mg/litre of culture supernatant). The water-soluble enzyme was purified close to protein homogeneity with a yield of 75 %. It requires the presence of 0.5 m-NaCl to remain soluble. It is indistinguishable from the detergent-extract wild-type enzyme with respect to molecular mass, thermostability, transpeptidase activity and penicillin-binding capacity
The transpeptidation reaction performed by the membranes of Streptomyces strain R61 fits the general...
The N-terminal region of the Streptomyces K15 DD-peptidase/penicillin-binding protein shows high hom...
The circular dichroism of the DD-carboxypeptidase-transpeptidase from Streptomyces R61 shows in the ...
Though synthesized with a cleavable signal peptide and devoid of membrane anchors, the 262-amino-aci...
The membrane-bound, 26000-Mr penicillin-binding protein of Streptomyces K15 has been isolated in the...
The membrane-bound, 26 000-Mr penicillin-binding protein of Streptomyces K15 has been isolated in th...
Streptomyces R61 and S. rimosus have an atypical penicillin-binding protein (PBP) pattern characteri...
The serine DD-transpeptidase/penicillin-binding protein of Streptomyces K15 catalyzes peptide...
The Km, app. values of the membrane-bound transpeptidase of Streptomyces R61 for the donor Ac2-L-Lys...
The 262 amino acid residue long DD-transpeptidase/penicillin-binding protein of Streptomyces K15 has...
Streptococcus faecalis ATCC 9790 possesses seven membrane-bound penicillin-binding proteins. They ha...
Kinetics and optical studies of Streptomyces DD-carboxypeptidases-transpeptidases led to the conclus...
The ability of the water-soluble DD-carboxypeptidases of Streptomyces strains albus G, R61, Kll and ...
A procedure allowing the purification of milligram amounts of the exocellular dd-carboxypeptidase-tr...
Streptococcus faecalis ATCC 9790 possesses six membrane-bound, penicillin-binding proteins. That num...
The transpeptidation reaction performed by the membranes of Streptomyces strain R61 fits the general...
The N-terminal region of the Streptomyces K15 DD-peptidase/penicillin-binding protein shows high hom...
The circular dichroism of the DD-carboxypeptidase-transpeptidase from Streptomyces R61 shows in the ...
Though synthesized with a cleavable signal peptide and devoid of membrane anchors, the 262-amino-aci...
The membrane-bound, 26000-Mr penicillin-binding protein of Streptomyces K15 has been isolated in the...
The membrane-bound, 26 000-Mr penicillin-binding protein of Streptomyces K15 has been isolated in th...
Streptomyces R61 and S. rimosus have an atypical penicillin-binding protein (PBP) pattern characteri...
The serine DD-transpeptidase/penicillin-binding protein of Streptomyces K15 catalyzes peptide...
The Km, app. values of the membrane-bound transpeptidase of Streptomyces R61 for the donor Ac2-L-Lys...
The 262 amino acid residue long DD-transpeptidase/penicillin-binding protein of Streptomyces K15 has...
Streptococcus faecalis ATCC 9790 possesses seven membrane-bound penicillin-binding proteins. They ha...
Kinetics and optical studies of Streptomyces DD-carboxypeptidases-transpeptidases led to the conclus...
The ability of the water-soluble DD-carboxypeptidases of Streptomyces strains albus G, R61, Kll and ...
A procedure allowing the purification of milligram amounts of the exocellular dd-carboxypeptidase-tr...
Streptococcus faecalis ATCC 9790 possesses six membrane-bound, penicillin-binding proteins. That num...
The transpeptidation reaction performed by the membranes of Streptomyces strain R61 fits the general...
The N-terminal region of the Streptomyces K15 DD-peptidase/penicillin-binding protein shows high hom...
The circular dichroism of the DD-carboxypeptidase-transpeptidase from Streptomyces R61 shows in the ...