Ultrasound intensification suppresses the need of methanol excess during the biodiesel production with Lipozyme TL-IM

The synthesis of biodiesel from sunflower oil and methanol based on transesterification using the immobilized lipase from Thermomyces lanuginosus (Lipozyme TL-IM) has been investigated under silent conditions and under an ultrasound field. Ultrasound assisted process led to reduced processing time and requirement of lower enzyme dosage. We found for the first time that ratio 1:3 (oil to methanol) was favoured for the ultrasound assisted enzymatic process which is lower as that favoured for the silent process (ratio of 1.4). Our results indicate that intensification provided by ultrasound suppresses the need of the excess of the methanol reactant during the enzymatic biodiesel production. Ultrasound assisted enzymatic biodiesel production is therefore a faster and a cleaner processes.The authors acknowledge Brenntag India Pvt. Ltd. for kindly providing gift sample of lipase enzyme Lipozyme TL IM to carry out the research work. All authors acknowledge the funding of Department of Science and Technology and Portuguese Science Foundation under the Indo-Portuguese collaborative program