Studies on the biocompatibility of bacterial cellulose

Bacterial cellulose was functionalized with a chimeric protein containing a cellulose-binding module and the adhesion peptide Arg-Gly-Asp. Small-diameter bacterial cellulose membranes were produced and subcutaneously implanted in sheep for 1–32 weeks. The implants triggered a biological response similar to other high surface-to-volume implants. There were no significant differences in the inflammation degree between the bacterial cellulose coated with the recombinant protein Arg-Gly-Asp–cellulose-binding module and the native bacterial cellulose. The implants were considered to be mildly irritating to the tissue compared to the negative control sample (expanded polytetrafluoroethylene). The analysis of the fluorescence microscopy revealed that, apart from increasing cell adhesion, the presence of Arg-Gly-Asp stimulated an even cell distribution, while the cells on the untreated bacterial cellulose seemed to form aggregates. Furthermore, the cells on the Arg-Gly-Asp–treated bacterial cellulose presented a more elongated morphology. Mechanical tests indicated that the small-diameter bacterial cellulose tubes were more elastic than the human arteries and veins.This work is funded by FEDER Funds through the Operational Programme for Competitiveness Factors COMPETE and National Funds through FCT - Foundation for Science and Technology under the Strategic Project PEst-C/AGR/UI0115/2011, the project PTDC/EBB-EBI/112170/2009 and under the PhD grant reference SFRH/BD/64838/2009. This work was also supported by Fundacao para a Ciencia e Tecnologia (FCT), Ministerio da Educacao e da Ciencia, Portugal, through the research Project PTDC/DES/104036/2008 and by QREN No. 1372 para Criacao de um Nucleo I&DT para Desenvolvimento de Produtos nas Areas de Medicina Regenerativa e de Terapias Celulares - Nucleo Biomat & Cell.Fabia K. Andrade is the recipient of a fellowship from Coordenacao de Aperfeicoamento de Pessoal de Nivel Superior (CAPES, Brazil). Fabia K Andrade and Nuno Alexandre contributed equally to this work