IN VITRO BIO-MEDICALSTUDIES ON PSIDIUM GUAJAVA LEAVES

Distinctive parts of guava Psidium guajava L. (PG) are a great wellspring of strong and intense bio medications. The present investigation was intended to extract and to identify the bioactive component of PG leaves, utilizing HPLC and assessed the activity of ethanol(70%) extract against Helicobacter pyroli, tuberculosis, diabetic (Alpha-glucosidase inhibitory %), arthritic (albumin denaturation inhibition) and aging (Anti-Collagenase) factorials. The outcomes of HPLC identification phenolic compounds showed that ethanolic extract contained gallic acid, caffeic acid, ferulic acid, cinnamic acid and they have highest values (4.71, 4.37, 3.82, 3.55 and 3.49µg/mg, respectively followed by resorcinol, chlorogenic, syringic acid and resormarinic acid (3.09, 2.93, 2.85 and 2.49µg/mg, respectively), while flavonoids were quercetin, hesperetin, kaempferol, quercitrin and rutin, where they presented 8.94, 7.61, 7.55, 7.13 and 6.37 µg/mg, respectively followed by catechin (5.12µg/mg) and apigenin (4.83 µg/mg). The identified alkaloids were corilagin, kaempfertin, and isoquinoline and they have values 2.13, 1.89 and 1.24µg/mg, respectively. Evaluation of inhibitory effect of PGL extract stated that PGL scored the highest values for effective concentrations (125 µg/mg) and MIC90 (26.6) comparing with activity of clarithromycin (C) (1.95 µg/mg and MIC90 (0.7), respectively against Helicobacter pyroli activity and 7.81µg/mg and MIC90 11.94 comparing with isoniazid standard (IS) at concentration 0.24µg/mg and MIC90 0.4 against tuberculosis activity. The IC50 of PGL against alpha-glucosidase activity comparing with values of Acarbose (A) showed that inhibitory percentage was lower at 1000µg/mg (79.22) than the value of acarbose (90.10) at the same concentration, while IC50 of PGL was higher (46.6) than that of acarbose (30.57). Percentage of albumin denaturation inhibition at 1000 µg/mg of both PGL extract and DSS were 71.34 and 89.35µg/mg, respectively, while IC 50 of both were 50.26 and 15.12, respectively. Collagenase inhibition percentage of PGL recorded 82.34 but EGCGrecorded 93.24 at concentration 1000 µg/mg. The IC50of PGL was higher (105.3) than the value of EGCG (40.3)