Data for: Inhibition is a prevalent mode of activity in the neocortex around awake hippocampal ripples in mice

Coordinated peri-ripple activity in the hippocampal-neocortical network is essential for mnemonic information processing in the brain. Hippocampal ripples likely serve different functions in sleep and awake states. Thus, the corresponding neocortical activity patterns may differ in important ways. We addressed this possibility by conducting voltage and glutamate wide-field imaging of the neocortex with concurrent hippocampal electrophysiology in awake mice. Contrary to our previously published sleep results, deactivation and activation were dominant in post-ripple neocortical voltage and glutamate activity, respectively, especially in the agranular retrosplenial cortex (aRSC). Additionally, the spiking activity of aRSC neurons, estimated by two-photon calcium imaging, revealed the existence of two subpopulations of excitatory neurons with opposite peri-ripple modulation patterns: one increases and the other decreases firing rate. These differences in peri-ripple spatiotemporal patterns of neocortical activity in sleep versus awake states might underlie the reported differences in the function of sleep versus awake ripples.This dataset could be opened in MATLAB. The VSFP and iGluSnFR datasets are organized in matrices whose dimensions correspond to ripple by time (1 second before and after ripple centers). The calcium and deconvolved traces in 2P calcium dataset are organized in neuron by time (3 seconds before and after ripple centers) by ripples format. For the VSFP datasets, the imaging and electrophysiology sampling rates are 100 and 2000 Hz, respectively. For the iGluSnFR dataset, the imaging and electrophysiology sampling rates are 100 and 1000 Hz, respectively. For the 2P calcium dataset, the imaging and electrophysiology sampling rates are reported inside the dataset's file. For ROI names, please refer to https://doi.org/10.7554/eLife.51972.Funding provided by: Natural Sciences and Engineering Research Council of CanadaCrossref Funder Registry ID: http://dx.doi.org/10.13039/501100000038Award Number: 40352Funding provided by: Alberta Prion Research InstituteCrossref Funder Registry ID: http://dx.doi.org/10.13039/501100021730Award Number: 43568Funding provided by: Canadian Institutes of Health ResearchCrossref Funder Registry ID: http://dx.doi.org/10.13039/501100000024Award Number: 39093