PECULIARITIES OF MINERAL EXCHANGE OF LIVER AND STRUCTURE OF THE MESENTERIAL LYMPH NODE OF ADOLESCENT RATS IN CONDITIONS OF LEAD CHRONIC INTOXICATION

The purpose of the research was to detect structural alterations of the mesenteric lymph node of male rats-adolescents taking into account changes in the mineral metabolism against a background of chronic intoxication with lead acetate. Material and methods. The experiment was carried out on 4-week male adolescents Wistar rats (10 rats) receiving per os lead acetate solution at a daily dose of 10 mg / kg body weight for 21 days, animals of the control group (10 rats) received food without heavy metal. The mesenteric lymph node and liver were examined (staining with hematoxylin and eosin, azur II and eosin) by light microscopy and morphometry; bioelement content in the liver was determined using atomic emission spectrometry with inductively coupled argon plasma. The non-parametric Mann - Whitney U criterion and the Pearson criterion χ² were used as statistical methods Results and discussion. The lymph node changed from an intermediate (in control) into a fragmented functional type that promotes active lymph flow through the sine system of the lymphoid organ. The ratio of T-dependent functional zones of the lymph node decreases. The increase in lead concentration in liver tissues was detected in 100 % of the observations. In 60 % of liver samples, a decrease in the content of potassium and zinc was detected. In 40% cases there was manganese exchange disorder, in 20 % there were deviations in calcium, iodine, phosphorus and silicon amounts. Thus, the chronic effect of lead acetate subacute doses on the body of rat male adolescents results in pronounced shifts in the exchange of bioelements. Against the background of altered biochemical processes, structural and functional rearrangements are formed in the mesenteric lymph node: depression of the immune processing of lymph, activation of the transport function of the organ