Comparative effects of MCB on reproduction and healthspan of <i>C</i>. <i>elegans</i>.

(a) Body length based on relative axial length (Time of flight, TOF) of worms fed E. coli OP50 versus MCB either alive or inactivated for the indicated time points. Each point represents an individual worm. (b) Body size of worms fed E. coli OP50 versus MCB either alive or inactivated. Each point represents an individual worm. (c) Brood size of worms fed E. coli OP50 versus MCB either alive or inactivated. 30 worms were tested individually per group. (d) Percentage of worms showing a Bag phenotype over the reproductive period in worms fed with E. coli OP50 or MCB either alive or inactivated. Data represent the mean ± SEM values on 35 worms/replicate for a total of 105 worms. (e) Percentage of worms showing an Avid phenotype over the reproductive period in worms fed with E. coli OP50 or MCB either alive or inactivated. Data represent the mean ± SEM values on 35 worms/replicate for a total of 105 worms. Statistical comparisons by 2 x 2 ANOVA. ns, no significant. (g) Survival curves of worms fed on E. coli OP50 versus MCB either alive or inactivated. n = 150 per group, see also S4 Table in S1 File).