Ca²⁺ supplementation <i>in vitro</i> has no impact on the production of some cytokines by mouse γδ T and B cells.

(a-c) Splenocytes from C57BL/6 mice were stimulated 4 h with 50 ng/ml PMA and 1 μg/ml ionomycin in either normal RPMI1640 medium (RPMInorm) or RPMI1640 medium supplemented with 1 mM Ca2+ (RPMIsuppl). The production of (a) IL-10 and (b) IL-17A by γδ T cells (live CD19/CD45R- CD4- CD8α- CD3ε+ γδTCR+ cells) and the production of (c) IFNγ by B cells (live CD3ε- CD4- CD8α- CD19/CD45R+ cells) was analysed by ICCS. Summary graphs (left panels) and representative data (right panels) from gated γδ T cells and B cells are shown, respectively. Data were pooled from three independent experiments with three mice per group per experiment (n = 9). (TIF)